Quantcast
Channel: ExpertCytometry
Browsing all 298 articles
Browse latest View live

Image may be NSFW.
Clik here to view.

What Is The International Cytometry Certification Exam (ICCE) And How To Pass It

Written by James McCracken, Ph.D. Clinical workers in flow cytometry and other fields have had certification requirements for quite some time. However, scientists in the basic research space have...

View Article


Image may be NSFW.
Clik here to view.

How To Use Flow Cytometry To Correctly Define T Cell Subsets And Their Functions

Written by Jennifer Snyder-Cappione, Ph.D. Flow Cytometry is a remarkably powerful tool for the study of T cells. It has been successfully used for many decades to accurately visualize and enumerate a...

View Article


Image may be NSFW.
Clik here to view.

How Droplets Are Charged And Drop Delays Are Determined During An...

Written by Michael Kissner. They way that droplets are charged is one of the more counterintuitive and complex aspects of cell sorting. My own experience as a past fledgling cytometrist certainly...

View Article

Image may be NSFW.
Clik here to view.

Why You Need To Use FMO Controls For All Multicolor Flow Cytometry Experiments

Written by James McCracken, Ph.D. What are the three most important parts of any flow cytometry experiment? Controls, controls, controls. Of course, this is mostly in jest. Mostly. Reagent selection,...

View Article

Image may be NSFW.
Clik here to view.

What Is Photon Counting And How To Use 8-Peak Rainbow Beads

Written by Michael Kissner For decades, flow cytometry professionals have been using 8-peak quality control beads to quickly check PMT sensitivity, resolution, and linearity on their flow cytometry and...

View Article


Image may be NSFW.
Clik here to view.

3 Reagents For Identifying Live, Dead, And Apoptotic Cells By Flow Cytometry

Written by James McCracken, Ph.D. As cells die, the membrane becomes permeable. This allows for antibodies to penetrate the cells, which can now mimic live cells. For this and other reasons, it’s...

View Article

Image may be NSFW.
Clik here to view.

How Cell Culture Medium Can Decrease Cell Viability During A Flow Cytometry...

By Michael Kissner When setting up a cell sorting experiment, there are many things to consider. You must consider which controls you’re going to use, which quality control beads are ideal, how you’re...

View Article

Image may be NSFW.
Clik here to view.

What Is The Coulter Principle And Why You Need To Obtain Complete Blood...

Written by James McCracken, Ph.D. When you get your blood drawn for your annual physical, or if you have been hospitalized, there are some common flow cytometry tests that your Medical Technician will...

View Article


Image may be NSFW.
Clik here to view.

Increase Cell Viability With These 3 Flow Cytometry Experimental Research...

By Michael Kissner The cell sorting process, while generally well-tolerated by most cell types, is inherently stressful. Cells are first manipulated in suspension for up to several hours to prepare and...

View Article


Image may be NSFW.
Clik here to view.

How To Differentiate T-Regulatory Cells (Tregs) By Flow Cytometry

Written by Jennifer Snyder-Cappione, Ph.D. T-regulatory cells (Tregs), formerly known as T suppressor cells, are a T cell subset with direct roles in both autoimmunity and responses to pathogens. Tregs...

View Article

Image may be NSFW.
Clik here to view.

5 Experimental Errors That Prevent Your Flow Cytometry Data From Passing The...

Written by Tim Bushnell, Ph.D. Reproducibility is a critical component of the scientific process. One cannot publish data if the experiments cannot be replicated. Unfortunately, as Begley and Ellis...

View Article

Image may be NSFW.
Clik here to view.

What Is MIFlowCyt And The FlowRepository, Or Why Flow Cytometry Is Being...

Written by Ryan Brinkman, Ph.D. Flow cytometry is a powerful screening tool that can be used to help identify target candidates on phenotypically defined cell populations. There are a myriad of...

View Article

Image may be NSFW.
Clik here to view.

Top Flow Cytometry Instrument, Reagent, And Software Trends To Pay Attention...

Written by Tim Bushnell, Ph.D. From instruments, to reagents, to software there are many flow cytometry innovations to pay attention to this year. These are just a few highlights of the great things...

View Article


Image may be NSFW.
Clik here to view.

What Is A Statistical Analysis T-Test And How To Perform One Using Flow...

Written by Tim Bushnell, Ph.D. Designing an antibody panel and running samples on a flow cytometer are not the only steps in a flow cytometry experiment. After you run your experiment, you have to...

View Article

Image may be NSFW.
Clik here to view.

What Is Flow Cytometry Light Scatter And How Cell Size And Particle Size...

Written by Mike Kissner Light scatter is unfortunately one of the more misunderstood concepts in cytometry. When you first learn flow cytometry, you were likely told the misleading phrase, “Forward...

View Article


Image may be NSFW.
Clik here to view.

3 Flow Cytometry Gates That Will Improve The Accuracy Of Your FACS Data Analysis

Written by Tim Bushnell, Ph.D. Flow cytometry data analysis typically involves a step where a series of gates are defined to identify the population of interest. The development of robust gating...

View Article

Image may be NSFW.
Clik here to view.

What Is The Coulter Principle And Why You Need To Obtain Complete Blood...

Written by James McCracken, Ph.D. When you get your blood drawn for your annual physical, or if you have been hospitalized, there are some common flow cytometry tests that your Medical Technician will...

View Article


Image may be NSFW.
Clik here to view.

Increase Cell Viability With These 3 Flow Cytometry Experimental Research...

By Michael Kissner The cell sorting process, while generally well-tolerated by most cell types, is inherently stressful. Cells are first manipulated in suspension for up to several hours to prepare and...

View Article

Image may be NSFW.
Clik here to view.

How To Differentiate T-Regulatory Cells (Tregs) By Flow Cytometry

Written by Jennifer Snyder-Cappione, Ph.D. T-regulatory cells (Tregs), formerly known as T suppressor cells, are a T cell subset with direct roles in both autoimmunity and responses to pathogens. Tregs...

View Article

Image may be NSFW.
Clik here to view.

5 Experimental Errors That Prevent Your Flow Cytometry Data From Passing The...

Written by Tim Bushnell, Ph.D. Reproducibility is a critical component of the scientific process. One cannot publish data if the experiments cannot be replicated. Unfortunately, as Begley and Ellis...

View Article
Browsing all 298 articles
Browse latest View live