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What Is The International Cytometry Certification Exam (ICCE) And How To Pass It
Written by James McCracken, Ph.D. Clinical workers in flow cytometry and other fields have had certification requirements for quite some time. However, scientists in the basic research space have...
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How To Use Flow Cytometry To Correctly Define T Cell Subsets And Their Functions
Written by Jennifer Snyder-Cappione, Ph.D. Flow Cytometry is a remarkably powerful tool for the study of T cells. It has been successfully used for many decades to accurately visualize and enumerate a...
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How Droplets Are Charged And Drop Delays Are Determined During An...
Written by Michael Kissner. They way that droplets are charged is one of the more counterintuitive and complex aspects of cell sorting. My own experience as a past fledgling cytometrist certainly...
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Why You Need To Use FMO Controls For All Multicolor Flow Cytometry Experiments
Written by James McCracken, Ph.D. What are the three most important parts of any flow cytometry experiment? Controls, controls, controls. Of course, this is mostly in jest. Mostly. Reagent selection,...
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What Is Photon Counting And How To Use 8-Peak Rainbow Beads
Written by Michael Kissner For decades, flow cytometry professionals have been using 8-peak quality control beads to quickly check PMT sensitivity, resolution, and linearity on their flow cytometry and...
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3 Reagents For Identifying Live, Dead, And Apoptotic Cells By Flow Cytometry
Written by James McCracken, Ph.D. As cells die, the membrane becomes permeable. This allows for antibodies to penetrate the cells, which can now mimic live cells. For this and other reasons, it’s...
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How Cell Culture Medium Can Decrease Cell Viability During A Flow Cytometry...
By Michael Kissner When setting up a cell sorting experiment, there are many things to consider. You must consider which controls you’re going to use, which quality control beads are ideal, how you’re...
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What Is The Coulter Principle And Why You Need To Obtain Complete Blood...
Written by James McCracken, Ph.D. When you get your blood drawn for your annual physical, or if you have been hospitalized, there are some common flow cytometry tests that your Medical Technician will...
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Increase Cell Viability With These 3 Flow Cytometry Experimental Research...
By Michael Kissner The cell sorting process, while generally well-tolerated by most cell types, is inherently stressful. Cells are first manipulated in suspension for up to several hours to prepare and...
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How To Differentiate T-Regulatory Cells (Tregs) By Flow Cytometry
Written by Jennifer Snyder-Cappione, Ph.D. T-regulatory cells (Tregs), formerly known as T suppressor cells, are a T cell subset with direct roles in both autoimmunity and responses to pathogens. Tregs...
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5 Experimental Errors That Prevent Your Flow Cytometry Data From Passing The...
Written by Tim Bushnell, Ph.D. Reproducibility is a critical component of the scientific process. One cannot publish data if the experiments cannot be replicated. Unfortunately, as Begley and Ellis...
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What Is MIFlowCyt And The FlowRepository, Or Why Flow Cytometry Is Being...
Written by Ryan Brinkman, Ph.D. Flow cytometry is a powerful screening tool that can be used to help identify target candidates on phenotypically defined cell populations. There are a myriad of...
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Top Flow Cytometry Instrument, Reagent, And Software Trends To Pay Attention...
Written by Tim Bushnell, Ph.D. From instruments, to reagents, to software there are many flow cytometry innovations to pay attention to this year. These are just a few highlights of the great things...
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What Is A Statistical Analysis T-Test And How To Perform One Using Flow...
Written by Tim Bushnell, Ph.D. Designing an antibody panel and running samples on a flow cytometer are not the only steps in a flow cytometry experiment. After you run your experiment, you have to...
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What Is Flow Cytometry Light Scatter And How Cell Size And Particle Size...
Written by Mike Kissner Light scatter is unfortunately one of the more misunderstood concepts in cytometry. When you first learn flow cytometry, you were likely told the misleading phrase, “Forward...
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3 Flow Cytometry Gates That Will Improve The Accuracy Of Your FACS Data Analysis
Written by Tim Bushnell, Ph.D. Flow cytometry data analysis typically involves a step where a series of gates are defined to identify the population of interest. The development of robust gating...
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What Is The Coulter Principle And Why You Need To Obtain Complete Blood...
Written by James McCracken, Ph.D. When you get your blood drawn for your annual physical, or if you have been hospitalized, there are some common flow cytometry tests that your Medical Technician will...
View ArticleClik here to view.
Increase Cell Viability With These 3 Flow Cytometry Experimental Research...
By Michael Kissner The cell sorting process, while generally well-tolerated by most cell types, is inherently stressful. Cells are first manipulated in suspension for up to several hours to prepare and...
View ArticleClik here to view.
How To Differentiate T-Regulatory Cells (Tregs) By Flow Cytometry
Written by Jennifer Snyder-Cappione, Ph.D. T-regulatory cells (Tregs), formerly known as T suppressor cells, are a T cell subset with direct roles in both autoimmunity and responses to pathogens. Tregs...
View ArticleClik here to view.
5 Experimental Errors That Prevent Your Flow Cytometry Data From Passing The...
Written by Tim Bushnell, Ph.D. Reproducibility is a critical component of the scientific process. One cannot publish data if the experiments cannot be replicated. Unfortunately, as Begley and Ellis...
View Article