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What Is MIFlowCyt And The FlowRepository, Or Why Flow Cytometry Is Being...
Written by Ryan Brinkman, Ph.D. Flow cytometry is a powerful screening tool that can be used to help identify target candidates on phenotypically defined cell populations. There are a myriad of...
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Top Flow Cytometry Instrument, Reagent, And Software Trends To Pay Attention...
Written by Tim Bushnell, Ph.D. From instruments, to reagents, to software there are many flow cytometry innovations to pay attention to this year. These are just a few highlights of the great things...
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What Is A Statistical Analysis T-Test And How To Perform One Using Flow...
Written by Tim Bushnell, Ph.D. Designing an antibody panel and running samples on a flow cytometer are not the only steps in a flow cytometry experiment. After you run your experiment, you have to...
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What Is Flow Cytometry Light Scatter And How Cell Size And Particle Size...
Written by Mike Kissner Light scatter is unfortunately one of the more misunderstood concepts in cytometry. When you first learn flow cytometry, you were likely told the misleading phrase, “Forward...
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3 Flow Cytometry Gates That Will Improve The Accuracy Of Your FACS Data Analysis
Written by Tim Bushnell, Ph.D. Flow cytometry data analysis typically involves a step where a series of gates are defined to identify the population of interest. The development of robust gating...
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How To Compensate A 4-Color Flow Cytometry Experiment Correctly
Written by Tim Bushnell, Ph.D. Compensation in flow cytometry is a critical step to ensure accurate interpretation of data. It is also one of the areas that’s steeped in mystery, myths and...
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12 Flow Cytometry Terms And Definitions Most Scientists Get Wrong
Written by Tim Bushnell, Ph.D. The most important part of executing a flow cytometry experiment correctly is actually understanding what you are doing. This means you must understand the terms and...
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The Difference Between Purity, Single Cell, And Recovery Cell Sorting Techniques
Written by Michael Kissner If you have experience sorting different kinds of cell types on a droplet sorter, you may have noticed that sorting efficiency often seems closely tied to the cell and sample...
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4 Spectral Viewers You Should Be Using For Your Flow Cytometry Experiments
Written by Tim Bushnell, PhD At the heart of flow cytometry is the ability to make meaningful measurements of fluorescently tagged cells. These fluorochromes can be bound to antibodies, a fluorescent...
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4 Critical Components In Cellular Proliferation Measurement
Written by Tim Bushnell, PhD Cell proliferation is a critical component in biological systems. While normal cell proliferation keeps the body functioning, abnormal proliferation (such as in cancer) can...
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4 Core Techniques For Improving Fluorescence Activated Cell Sorting Results
Written by Mike Kissner. Cell sorters have become more sophisticated to rival the multicolor capabilities of analytical cytometers, offering up to seven lasers and a score or more of detectors....
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How To Analyze FACS Data And Prepare Flow Cytometry Figures For Scientific...
Written by Tim Bushnell, PhD “It would be possible to describe everything scientifically, but it would make no sense; it would be without meaning, as if you described a Beethoven symphony as a...
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How To Create A Flow Cytometry Quality Assurance Protocol For Your Lab
By EditaMotyčáková, Ph.D. Editor’s note: This is based on Edita’s experiences implementing the proposals reported in Perfetto et al., (2012) Nature Protocols 7:2067. She submitted this to the ExCyte...
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5 Gating Strategies To Get Your Flow Cytometry Data Published In...
Written by Tim Bushnell, PhD “Every block of stone has a statue inside it and it is the task of the sculptor to discover it.” — Michelangelo When sitting down to perform a new analysis of flow...
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4 Biggest Mistakes Scientists Make During Multicolor Flow Cytometry Cell...
Written By Mike Kissner Multicolor cell sorting is a complicated process and certain scientific errors can be common. Unsuccessful multicolor sorts can result in erroneous data and inconclusive...
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What Is Fluorescent Activated Cell Sorting And 4 Other Questions About FACS...
Written By Tim Bushnell, PhD Prior to the mid-1960’s, the ability to study a defined cell type was severely limited. Researchers had to use centrifugation methods, such as differential centrifugation,...
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How To Improve Reproducibility Through The Automated Analysis Of Flow...
Written by Ryan Brinkman, Ph.D. Editor’s Note: Reproducibility continues to be a critical area that all researchers need to be aware of. From the NIH’s focus on reproducibility in grant applications,...
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4 Gating Controls Your Flow Cytometry Experiment Needs To Improve...
Written by Tim Bushnell, PhD To reproduce reliably in flow cytometry, one must control the gate. The identification of the target cells of an experiment is the critical first step to performing the...
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What Is A Flow Cytometry Laser And How Flow Cytomtery Optics Function
Written by Tim Bushnell, PhD The optical system of a flow cytometer consists of an elegant coordination of many components that function concordantly and synchronously to generate the signals that we...
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How Flow Cytometry Optical System Components Work
Written by Tim Bushnell, Ph.D. The importance of the optical system of your flow cytometer was established in Part I of this series, noting in particular the benefits of foundational knowledge to gain...
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