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How To Do Phospho-Flow Cytometry
I often have researchers come into the core wanting to look at the activation and downstream signaling events that occur in different immune cells. These events occur in response to signals such as...
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Fluorescence Funny Business
When we learn about fluorescence, the first thing we are told is that fluorophores emit photons that are higher wavelength than the photons that they absorb. What this specifically refers to is the...
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7 Tips For Measuring And Reporting Apoptosis By Flow Cytometry
Cell death is a fact of biological life. How, when, where and most importantly, why cells die, can have huge biological consequences on the path an organism may take. Apoptosis, or programed cell...
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6 Tips For Applying The Right Statistical Test To Your Flow Cytometry Data
Flow cytometry data are numbers rich. Data from experiments can be population measurements (percent of CD4+ cells, for example), or it can be expression level (median fluorescent expression of CD69 on...
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8 Time-Saving FACSDiva Software Tips
BD Biosciences brand of digital flow cytometers, including the FACSCanto, the LSR-II, FACSAria and Fortessa, utilize a software acquisition program known as FACSDiva. Diva is aptly named as it can be a...
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Top Flow Cytometry Instruments, Reagents & Software To Look Forward To In...
Here at ExCyte–we are excited for all of the new flow cytometry instruments, reagents, and software you will have access to in 2015. There are so many new and innovative products that we have been...
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The Most Common Mistake Researchers Make When Designing Flow Cytometry...
Pairing highly expressed antigens (like CD3) with dimmer fluorochromes, and the antigens of interest with the brightest fluorochromes, is a key part of panel design with few tools to help. With early...
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5 Flow Cytometry Errors Reviewers Despise
We all know that flow cytometry makes individual measurements on large populations of cells, it allows for statistical analysis of the data, lending strength to a researcher’s conclusions. Likewise,...
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Why You Should Never Manually Compensate Your Data
(Figure courtesy of Pratip K. Chattopadhyay, Ph.D.) One of the most important steps in proper flow cytometry is the process of compensation. There are a lot of rumors and mysteries that fill laboratory...
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How To Create The Right Flow Cytometry Antibody Panel Every Time
Sudoku puzzles seem to be all the rage. I see it in coffeehouses, at the airport, even in doctors offices. Everyone is trying to work out how to fit the numbers into the grids so that everything adds...
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3 Experiments You Can Do Easier On An ImageStream Flow Cytometer
What happens if one combines the power and speed of traditional flow cytometers with the resolution of a microscope? Cytometry is the study of biological processes at the whole cell level and includes...
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Why Recovery, NOT Purity, Is The Best Measure Of A Cell Sorting Performance
(Written by Carol Oxford, ExCyte Founding Partner.) One of the best perks of being a part of the flow cytometry community is the opportunity to attend one of the many regional flow meetings. The chance...
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How To Perform A SPICE Analysis With FlowJo
Flow cytometry data analysis is getting more complex. Gone is the rule of 2-3 color experiments. Even beginners are starting with 5+ color assays, and the adoption of mass cytometry has the potential...
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6 Keys To Running A Proper Clinical Flow Cytometry Experiment
Clinical use of flow cytometry has paralleled the development of instrumentation and reagents. One early application for flow cytometry is the measurement of DNA content. Malignancies and neoplasms...
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When To Use (And Not Use) Flow Cytometry Isotype Controls
Antibodies can bind to cells in a specific manner – where the FAB portion of the antibody binds to a high-affinity specific target or the FC portion of the antibody binds to the FcR on the surface of...
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Why Understanding The Jablonski Diagram Will Help You Publish Your Flow...
We are all used to interruptions during our working day, from the ping of an email notification to the knock of a fellow researcher who wants to troubleshoot their experiment. Fortunately, most of...
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7 Advanced Flow Cytometry Data Analysis Tips For Multi-Color Experiments
There was a time when two- and three-color experiments were considered very complex flow cytometry experiments. A limited number of dyes and light sources meant a limited number of options overall. In...
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3 Types Of Flow Cytometry Beads That Will Help Get Your Data Published
When starting a flow cytometry experiment, you should begin with the end in mind. What data do you need to generate to support your other data? What data would really impress the reviewers in your...
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5 Important Peer Review Questions To Answer Before Submitting Your Flow...
Publish or perish remains the mantra of science. All the experiments and experience in the world do not count if you are unable to communicate your results to the scientific community. As part of that...
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How To Detect Microvesicles, Microparticles, And Ectosomes By Flow Cytometry
Written by Phil Hexley, Ph.D. There seems to be a big fuss about small flow these days. What is small flow? Microvescles, microparticles, ectosomes—the terminology is all over the place but one thing...
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