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4 Critical Rules For Spectral Unmixing
Spectral unmixing is the mathematical process by which a spectrum is broken down into the abundances of the different fluorochromes that make up the observed spectrum. This was described in the paper...
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A Basic Guide To Flow Cytometry (3 Foundational Concepts)
Mastering foundational concepts are imperative for successfully using any technique or system. Robert Heinlein introduced the term ‘Grok’ in his novel Stranger in a Strange Land. Ever since then it...
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5 Flow Cytometry Strategies That Sun Tzu Taught Me
Sun Tzu was a Chinese general and philosopher. His most famous writing is ‘The Art of War’, and has been studied by generals and CEOs, to glean ideas and strategies to help their missions. I was...
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What Star Trek Taught Me About Flow Cytometry
It is no secret that I am a very big fan of the Star Trek franchise. There are many good episodes and lessons explored in the 813+ episodes, 12 movies (and counting). Don’t worry, this blog is not...
View ArticleFlow Cytometry Year in Review: Key Changes To Know
Here we are, at the end of an eventful year 2021. But with the promise of a new year 2022 to come. It has been a long year, filled with ups and downs. It is always good to reflect on the past year as...
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The Fluorochrome Less Excited: How To Build A Flow Cytometry Antibody Panel
Fluorochrome, antibodies and detectors are important. The journey of a thousand cells starts with a good fluorescent panel. The polychromatic panel is the combination of antibodies and fluorochromes....
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3 Must-Have High-Dimensional Flow Cytometry Controls
Developments such as the recent upgrade to the Cytobank analysis platform and the creation of new packages such as Immunocluster are reducing the computational expertise needed to work with...
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5 Common Flow Cytometry Questions, Answered
I want to thank all of you who send us your questions about flow cytometry, so I thought I would dip into the old email bag and answer a few of the common ones here. If your question isn’t answered...
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Fickle Markers: Solutions For Antibody Binding Specificity Challenges
Reproducibility has been an ongoing, and important, concept in the sciences for years. In the area of biomedical research, the alarm was sounded by several papers published in the early 2010’s....
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The Power Of Spectral Viewers And Their Use In Full Spectrum Flow Cytometry
What photon from yonder fluorochrome breaks? It is … umm… hmmm. Let me see. Excitation off a 561 nm laser, with an emission maximum of 692 nm. I’m sure if Shakespeare was a flow cytometrist, he might...
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