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How To Perform A T-Test
With the ability to capture expression data at the single cell level through many thousands of cells in a short time, flow cytometry data is very numbers rich. The importance of those numbers and how...
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Critical Steps in DNA Cell Cycle Analysis
DNA cell cycle analysis is a very powerful technique in flow cytometry. It is deceptively easy, but there are several critical things to remember to ensure successful analysis. Collect enough events....
View ArticleTop Cell Sorter
The question always arises as to what is the top cell sorter on the market. This question is a difficult one to generalize because there are several considerations that need to be made in choosing a...
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Cell Cycle Analysis
Cell cycle analysis by flow cytometry uses a DNA binding dye, such as propidium iodide (PI), 7- aminoactinomycin D (7-AAD) or 4’,6-diamidino-2phenylindole (DAPI), to determine the cell cycle state of a...
View ArticleHyperlog Scaling
A variation on biexponential scaling similar to logicle scaling. The biexonential scale is a combination of linear and log scaling on a single axis using an arcsine function as its backbone....
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Cell Sorting
Cell Sorting is the process of isolating cells after identification of the cells using the principles of flow cytometry. The upstream components of the cell sorter are common to all flow cytometers....
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10 Things Smart Scientists Do Before Sorting Cells
Cell sorting can be a scary proposition. A precious sample is introduced into a machine that pressurizes the cells to 70 PSI, moves them past one or more lasers, vibrates the stream...
View ArticleCyTOF
Mass Cytometry, commercialized by the company DVS Sciences, in the instrument called the CyTOF is a newly emerging technology in the field of flow cytometry. This technology replaces traditional...
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Sorting
Cell sorting remains the best tool to isolate and purify cellular populations that can be phenotypically defined. This is especially true for rare-event detection and purification. Successful rare...
View ArticleFlow Cytometry Training
Flow cytometry can be an intimidating tool for the new cytometrist. There are many sources that one can turn to for training and education. Local Shared Resource Lab Manager – If you institute has a...
View ArticleHow To Do Flow Cytometry?
Flow cytometry is a powerful tool for asking and answering questions at the whole cell level. The first step in any flow cytometry experiment is to define the hypothesis or biological question that is...
View ArticleProliferation Experiments
Cell proliferation can readily be measured by flow cytometry. Depending on the research question, there are several different techniques that can be used. Cell counting experiments – this relatively...
View ArticleFlow Cytometry Education
As with all complex technology there are many different levels of education that users should avail themselves of. Basic instrument operation – this level of education is akin to learning how to drive...
View ArticleTop Flow Cytometer
What is the top flow cytometer? The easy answer is the flow cytometer that matches your needs and fits within your budget. However, before running off to spend cash, consider the following. What are...
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Counting Cells Will Save Your Flow Cytometry Experiment, If You Do It Like This
Fast, accurate or efficient – pick two. How to decide when you can’t have it all. The hemocytometer is considered the gold standard for cell counting. Invented by Louis Charles Malassez, this precision...
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4 Fluidics Tips That Will Change Your Flow Data For The Better
Friday is the 4th of July in the US – and we celebrate that day with picnics, spending time OUTSIDE the lab and fireworks. And our Independence, but I’m not up for a political discussion right now. For...
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Top 10 Flow Cytometry Resources
I’ve been in the world of flow cytometry and cell sorting for a very long time. Now, don’t worry, this isn’t going to be some lament about the “good old days.” Well, maybe just a little. But there will...
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10 FlowJo Version X Hacks That Will Help You Publish Your Flow Cytometry Data
So you just got the most amazing results of your life and you can wait to show it off in lab meeting, or create the figure for a publication. Here are a couple of tips that will help you ensure that...
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How To Design Accurate & Effective Flow Antibody Panels (or, What’s An OMIP?)
I was at a meeting talking about the principles of panel design. At the end of my talk, I had an investigator approach me and ask why he was not making progress on his 15-color panel that he started...
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5 Mistakes Scientists Make When Doing Flow Cytometry Proliferation Experiments
Measuring cell proliferation can be done in a number of ways. There is the below tried and true method of counting cells. This straightforward assay can help determine if the cells are proliferating...
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