Electrons
Counted by the Photomultiplier Tube (PMT) in the flow cytometer. Photons enter the PMT and the signal is amplified in the PMT when a photon strikes the anode and “knocks” of electrons. These electrons...
View ArticleLongpass Filter
A filter that allows light over a set wavelength to pass through and reflects light below the set wavelength. For example, a longpass filter with a wavelength of 670nm would allow light with a...
View ArticleShortpass Filter
A filter that allows light over a set wavelength to pass through and reflects light above the set wavelength. For example, a shortpass filter with a wavelength of 450nm would allow light with a...
View ArticleBandpass Filter
A filter that allows light between a set wavelength to pass through and reflects light above and below the set wavelength. For example, a bandpass filter with a wavelength of 550/40nm would allow light...
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ExCyte in Southern California
ExCyte In Association With SoCal Flow Flow Cytometry Core Principles Sunday, March 23rd, 2014 Flier And Course Outline Are Listed Below. Click On Images To Print.
View ArticleBlue Laser
The most common laser type in a flow cytometer. Typically, this laser has a wavelength of 488nm in flow cytometers. In fact, the term “Blue” laser is often interchanged with “488″ laser. Frequently...
View ArticleRed Laser
The second most common laser in a flow cytometer after the “blue” laser. The “red” laser typically has a wavelength of 633nm, but new flow cytometers are starting to use a “red” laser with a wavelength...
View ArticleViolet Laser
Another very common laser after the “blue” and “red” laser in flow cytometers. A “violet” laser in flow cytometry typically is referred to as the 405 because most flow cytometers use a violet laser...
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ExCyte in Cambridge
ExCyte In Association With The Babraham Flow Cytometry Facility Presents: Advanced Experimental Design -2 Day Course Thursday, February 20, 2014 AND Friday, February 21, 2014 Flier And Course...
View ArticleUV Laser
A laser with a wavelength in the UV range. Typically in flow cytometers, the UV laser has a wavelength of 350nm or 355nm. Some have a wavelength of 375nm.
View ArticleGreen Laser
The laser type in flow cytometers with a wavelength of around 530nm. Standard “green” lasers are about 532nm, but vary between 530nm and 535nm usually. The green and yellow laser are more effective at...
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How To Create Flow Cytometry Gates
After completing the perfect staining and cytometry run, the hard work begins – data analysis. To properly identify the cells of interest, it is critical to pull together knowledge of the biology with...
View ArticleYellow Laser
A laser type in a flow cytometer with a wavelength of about 560nm. The green and yellow laser are more effective at exciting PE and its tandems than the traditional blue laser. The yellow laser is also...
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ExCyte in Dublin
ExCyte In Association With The Flow Cytometry Facility At Trinity College Dublin And The Irish Cytometry Society Presents: Flow Cytometry Sorting -2 Day Course Thursday, February 27, 2014 AND...
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ExCyte in New Mexico
ExCyte In Association With The UNM Center For Biomedical Engineering Presents: Principles and Practices for Core Managers – 2 Day Course Thursday, March 20, 2014 AND Friday, March 21, 2014...
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ExCyte at UC Davis
ExCyte In Association With The UC Davis Flow Cytometry Shared Resource Laboratory Presents: Flow Cytometry Bootcamp Monday, March 3, 2014 Flier And Course Outline Are Listed Below. Click On...
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ExCyte in SoCal
ExCyte In Association SoCal Flow Presents: Flow Cytometry Core Principles Sunday, March 23, 2014 Flier And Course Outline Are Listed Below. Click On Images To Print.
View ArticleFive Important Tips For Analyzing Your Data
Depending on the experimental design, many researchers will be doing complex assays that will require statistical analysis to determine if the hypothesis being tested is statistically significant or...
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6 Flow Cytometry Gating Tips That Most Scientists Forget
After completing the perfect staining and cytometry run, the hard work begins – data analysis. To properly identify the cells of interest, it is critical to pull together knowledge of the biology...
View ArticleLogicle Scaling
An implementation of biexponential scaling published by the Herzenberg lab at Stanford. The biexonential scale is a combination of linear and log scaling on a single axis using an arcsine function as...
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