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Experimental Controls For Reproducible Flow Cytometry Measurements
With the increased focus on reproducibility of scientific data, it is important to look at how data is interpreted. To assist in data interpretation, the scientific method requires that controls are...
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Instrument Quality Control For Reproducible Flow Cytometry Experiments
The NIH has released a series of reproducibility guidelines that scientists must address. These guidelines have been introduced because there’s a lot of data showing that reproducibility in science is...
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The Need For Speed In Flow Cytometry Data Analysis
Speed is a highly touted metric in flow cytometry. Look at any vendor’s website and you will see the highlights on how many events per second their instrument can acquire, how many cells can be sorted...
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Best Practices In Flow Cytometry Compensation Methodologies
What is compensation and when should you do it? In this first of 3 blog articles, we will discuss the principles of compensation, as well as why it is important, and how to perform compensation....
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Reproducibility In Flow Cytometry Requires Correct Compensation
Why do we have to compensate flow cytometry data? Newcomers to flow cytometry are often confronted with one of the most confounding issues in flow cytometry. That is, trying to understand the whole...
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The Truth About Flow Cytometry Measurement Compensation
In most research labs, there exists a notebook that contains the tried and true protocols for lab members to follow. These hallowed, often coffee-stained, pages teach the researchers everything — from...
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Why Cell Cycle Analysis Details Are Critical In Flow Cytometry
The lifecycle of a cell can be described in stages. In diploid cells, much of the time they exist in a resting state, where a cell does what a cell does — such as, undergo differentiation. In some...
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6 Areas Of Consideration For Flow Cytometry Cell Cycle Analysis
As discussed previously, cell cycle assays require optimization of fixation and dye concentrations, but that is just the beginning. There are important considerations when performing the assay to...
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Best Flow Cytometry Cell Sorting Practices
Walk into any flow cytometry facility and you will see one or more cell sorters. These devices use the principles of flow cytometry to isolate phenotypically defined cells to a high degree of purity...
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From Purity To Biosafety, Understanding The Cell Sorting Process
The Power Of Flow Cytometry In Cell Sorting Often, cell sorting is described as trying to find a needle in a haystack. In 2008, David Brown wrote an article on cell sorting for the Washington Post. In...
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Using Begley’s Rules To Improve Reproducibility In Flow Cytometry
Written By: Tim Bushnell, Ph.D. Isaac Newton was famous for saying “If I have seen further than others, it is by standing upon the shoulders of giants.” Implicit in that statement is that the...
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3 Ways To Improve Flow Cytometry Troubleshooting
Written by: Tim Bushnell, Ph.D. I spend a lot of time working with my colleagues and clients retrospectively troubleshooting their data. This involves trying to understand and explain what might’ve...
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3 Questions You Should Be Asking About Flow Cytometry Controls For Your...
Written By: Tim Bushnell, Ph.D. What are the controls that you should be considering when planning and designing your flow cytometry experiments? Are you using isotype controls? Should you be? Isotype...
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3 Considerations To Ensure Your Cell Sorting Flow Cytometry Experiments Run...
Written By: Tim Bushnell, Ph.D. Cell sorting is such an important technique because it’s a gateway tool for many other downstream applications. Assays such as culturing cells, genomic analysis,...
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Microscopy – 5 Reasons Coverslips Are Important For High-Quality Imaging
Written By: Heather Brown Harding, Ph.D. Most people are familiar with coverslips being placed on slides to protect the sample, but that’s not the only reasons that coverslips are important. They also...
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3 Action Steps You Can Take Right Now To Improve Your Flow Cytometry...
Written By: Tim Bushnell, Ph.D. Reproducibility is a key issue in science. Massive amounts of time and money are wasted when the results of experiments are not reproducible. For example, I was called...
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5 Essential Controls For Reproducible Fluorescent Microscopy Imaging
Written By: Heather Brown Harding, Ph.D. Fluorescent imaging has the potential to bring great insights into your research project. But, there is a lot of room for error. And one of the most common and...
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3 Ways To Measure Cell Death With Flow Cytometry
Written By: Tim Bushnell, Ph.D. Cell death is a natural part of the lifecycle of a cell. In cases of development, it is critical for the shaping of fingers during human development. The processes of...
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Avoid Data Loss By Following These Steps To Set Your Flow Cytometry Gates...
Written By: Tim Bushnell, Ph.D. How are you defining your gates? When you do primary analysis or single tube analysis, it’s important that you make sure your gates are set correctly. You need to know...
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The Right Way To Read A Flow Cytometry Scientific Paper
Written By: Tim Bushnell, Ph.D. Scientific reproducibility and the public’s confidence in scientific results is critically important. One has to look no further than the website Retraction Watch to...
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