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The Difference Between Linear And Log Displays In Flow Cytometry
Written by Tim Bushnell, Ph.D Data display is fundamental to flow cytometry and strongly influences the way that we interpret the underlying information. One of the most important aspects of graphing...
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3 Advantages Of Using The ZE5 Cell Analyzer
Written by Tim Bushnell, Ph.D Since the first laser was mounted to create the first flow cytometer, there has been a push for more – more lasers, more detectors, and more colors. Why? So researchers...
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2 Key SPADE Parameters To Adjust For Best Flow Cytometry Results
Written by Tim Bushnell, Ph.D Mass cytometry panels routinely include 30 or more markers, but traditional analysis methods like bivariate gating can’t adequately parse the resulting high-dimensional...
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Planning For Surface Staining Of Cells In Flow Cytometry
Written by Tim Bushnell, Ph.D One of the most common assays in flow cytometry is the surface labeling of cells with antibodies. Often termed “immunophenotyping”, it allows the researcher to identify,...
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How To Perform A Flow Cytometry t-Test
Written by Tim Bushnell, Ph.D The ultimate goal of any experiment is to analyze data and determine whether it supports or disproves a given hypothesis. To do that, scientists turn to statistics....
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3 Ways The ZE5 Cell Analyzer Accelerates Flow Cytometry Research Opportunities
As new instruments come on the market, vendors are quick to provide data proving the systems’ prowess including sensitivity, speed, and such. These are important characteristics of the instrument, and...
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Measuring Receptor Occupancy With Flow Cytometry
Written by Tim Bushnell, Ph.D The field of medical therapeutics is moving into the area of precision medicine. In a global sense, precision medicine requires the doctor to assess a patient’s unique...
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5 Essential Calculations For Accurate Flow Cytometry Results
Flow cytometry is a numbers game. There are percentages of a population, fluorescence intensity measurements, sample averages, data normalization, and more. Many of these common calculations are...
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4 Considerations For Assessing Protein Phosphorylation Using Flow Cytometry
Signaling pathways are of great interest in many areas of research because dysregulation of these pathways can lead to diseases such as cancer and lupus. This also means these pathways contain...
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How to Perform Doublet Discrimination In Flow Cytometry
What is doublet discrimination? You are probably familiar with the term, “doublet discrimination” or “doublet exclusion”, and have likely included this flow cytometry measurement into at least some...
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5 Best Practices For Accurate Flow Cytometry Results
How do you follow best practices in flow cytometry to improve reproducibility? Reproducibility is in the science spotlight these days. With the growing body of evidence showing how much translational...
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Use This Preparation Guide For Accurate Flow Cytometry Results
A colleague related the following conversation/mini-rant that happened in their facility just the other day. The names have been changed to protect the innocent. New User #1: “Hi, I want to sort and...
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5 Essential Beads For Flow Cytometry Experiments
Flow cytometry is designed to measure physical and biochemical characteristics of cells and cell-like particles using fluorescence. Fundamentally, any single-particle suspension (within a defined size...
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How To Achieve Accurate Flow Cytometry Calcium Flux Measurements
Most flow cytometry experiments work with antibodies conjugated to a fluorochrome for some variation on immunophenotyping. However, any fluorochrome that is excited by one of the available excitation...
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How To Choose The Correct Antibody For Accurate Flow Cytometry Results
Next to the flow cytometer itself, the most important component of a flow cytometry experiment comes down to the antibodies. It is by using antibodies conjugated to fluorescent markers that we are...
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5 Considerations For Statistical Analysis Of Flow Cytometry Data
Congratulations, your grant has been funded! Now comes the hard part — performing the work that you are being funded to do. This means generating data and publishing papers. What was that hypothesis...
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3 Requirements For Accurate Flow Cytometry Compensation
For those new to flow cytometry, compensation is confusing at best and terrifying at worst. Likewise, those who have been doing flow cytometry since the analog ages may be holding on to practices...
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How to Optimize Flow Cytometry Hardware For Rare Event Analysis
“Not everything that can be counted counts and not everything that counts can be counted.” — William Bruce Cameron (but often misattributed to Albert Einstein) What does this quote mean in terms of...
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Procedural Limitations That Impact The Quality Of Rare Event Flow Cytometry
Having dealt with the hardware issues related to rare event analysis in the first part of this series, it is time to turn to our second focus: how samples are prepared. Stem cells, circulating tumor...
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Statistical Challenges Of Rare Event Measurements In Flow Cytometry
To conclude our series on rare event analysis, it is time to discuss the statistics behind rare event analysis. The first 2 parts of this series covered the hardware aspects of measuring rare events...
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