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5 Steps To Improve Your Flow Cytometry Data Analysis
Written By: Tim Bushnell, Ph.D. With the continued emphasis on improving the reproducibility of scientific data, it is critical to remember that there is no single step that will solve this problem....
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5 Special Considerations for Live Cell Imaging
Written By: Heather Brown-Harding, Ph.D. Live cell imaging is advantageous for research were you may be worried about artifacts of fixation or when you want to measure a phenomenon over time. Live...
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5 FlowJo Hacks To Boost The Quality Of Your Flow Cytometry Analysis
Written By: Tim Bushnell, Ph.D. Primary data analysis, that is the analysis at the sample or tube level, is where the populations of interest are identified and the necessary data is extracted for...
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4 Steps To Implementing a QC Program For Your Flow Cytometry Experiments
Written By: Tim Bushnell, Ph.D. With the emphasis on reproducibility in science, it is important to look at the process of quality control and quality assurance and how these can help improve the...
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7 Things You Didn’t Know About Imaging Cytometry
Written By: Tim Bushnell, Ph.D. We are visual creatures. We seek to capture and describe the world around us. Some of the earliest evidence of this is found in the cave paintings that have been found...
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6 Microscopy Assays To Determine Cell Health and Improve Your Experimental...
Written By: Heather Brown-Harding, Ph.D. When you’re performing imaging, we always want to make sure that any phenotype we see isn’t just an artifact of unhealthy cells or if you’re doing drug...
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Ask These 7 Questions Before Purchasing A Flow Cytometer
Written By: Tim Bushnell, Ph.D. Using funds to make a capital purchase can be an exciting time in a facility. If you don’t have the funds in hand, planning for future purchases requires due diligence...
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4 Ways To Analyze Tissues By Flow Cytometry
Written By: Tim Bushnell, Ph.D. Why are we interested in cytometry? What are its practical applications? Let’s start by defining cytometry as the measurement of cellular processes at the whole-cell...
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3 Components Of Every Flow Cytometer You Don’t Know Enough About
Written By: Tim Bushnell, Ph.D. All flow cytometer instruments have a certain 3 components you likely don’t know enough about. The way they are put together will dictate the performance of the system....
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Understanding Reproducibility in Flow Cytometry – It’s the Antibodies!
Written By: Tim Busnell, Ph.D. Reproducibility is key to the scientific method. After the results of a study are published, the community validates the findings and extends them. If the findings are...
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Use These 5 Techniques for Super Resolution
Written By: Heather Brown-Harding, Ph.D. When you need better resolution than what can be achieved using a traditional microscope, it can be very intimidating to figure out which microscopes will work...
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Mass Cytometry Revolves Around These 5 Things
Written By: Tim Busnell, Ph.D. 1. How it works 2. Panel design 3. Sample preparation 4. Data analysis 5. Imaging mass cytometry Today’s article will summarize the functionality of mass cytometry...
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We Tested 5 Major Flow Cytometry SPADE Programs for Speed – Here Are The Results
Written By: Tim Bushnell, Ph.D. As a follow-up to our post on tSNE where we compared the speed of calculation in leading software packages, let’s consider the case of SPADE (Spanning-tree Progression...
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7 Individual Artifacts In Fluorescence Microscopy And How To Minimize Them
Written By: Heather-Brown Harding, PhD There are 7 different “artifacts” that may be affecting the quality of your imaging. Before digging into the details, let’s begin by defining an artifact:...
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3 Compensation Mistakes That Will Ruin Your Flow Cytometry Experiments
Written By: Tim Bushnell, Ph.D. Putting it basically, compensation is the mathematical process of correcting spectral spillover from a fluorochrome into a secondary detector. Yet even the most...
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5 Techniques For Dramatic Improvements In Reproducibility
Written By: Heather Brown-Harding, PhD Image manipulation has become a major problem in science, whether intentional or accidental. This has exploded with the advent of digital imaging and software...
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5 Techniques For Dramatic Improvements In Reproducibility
Written By: Heather Brown-Harding, PhD Image manipulation has become a major problem in science, whether intentional or accidental. This has exploded with the advent of digital imaging and software...
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5-Point Guide To Buying A New Microscope For Your Lab
Written By: Heather Brown-Harding, PhD Have you ever noticed how painful it can be to purchase a new microscope? It would be hard to miss – this can be a frustrating process. A lot of scientists and...
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Discover The Myriad Applications Of Beads In Flow Cytometry
What is the single-most important feature of a flow cytometry experiment? Arguably, it’s the stained cells that gather data about biological processes of interest. However, a flow cytometer can...
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4 Flow Cytometry Assays For Monitoring Intracellular Processes
Written By: Tim Bushnell, PhD The most common flow assay is undoubtedly immunophenotyping, in which fluorescently tagged antibodies are used to bind to cellular proteins. This allows you to determine...
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